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M9650347.TXT
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1996-03-09
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Document 0347
DOCN M9650347
TI A rapid and inexpensive method for processing induced sputum for
detection of Pneumocystis carinii.
DT 9605
AU Rocha P; Awe RJ; Guy ES; Gaines V; Banez EI; Stager CE; Guntupalli KK;
Department of Pulmonary Medicine, Ben Taub General Hospital,; Houston,
TX 77030, USA.
SO Am J Clin Pathol. 1996 Jan;105(1):52-7. Unique Identifier : AIDSLINE
MED/96143465
AB The authors describe a method to process induced sputum specimens for
detection of Pneumocystis carinii which is simple, rapid and
inexpensive. Induced sputum and bronchoalveolar lavage (BAL) were
obtained within a 24-hour period from 41 patients who were HIV-positive
and had pulmonary symptoms suspicious for P carinii pneumonia. Induced
sputum or BAL fluid was placed into Saccomanno's fixative, blended, and
centrifuged. The sediment was stained for P carinii cysts by a modified
method with Fungi-Fluor Solution A (Polysciences, Warington, PA) and the
Genetic Systems Pneumocystis carinii Immunofluorescence Antibody
(Genetic Systems, Seattle, WA). The Genetic Systems stain on the BAL
specimen was positive in 35 patients and was the standard for
comparison. With a single induced sputum, the Genetic Systems stain
detected 31 (89%) positive patients, whereas the Fungi-Fluor stain
detected 21 (60%). The sensitivity for detecting P carinii cysts in
induced sputum was significantly greater (P < 0.05) for the Genetic
Systems stain.
DE AIDS-Related Opportunistic Infections/*DIAGNOSIS Bronchoalveolar Lavage
Fluid/MICROBIOLOGY Comparative Study Fixatives Fluorescent Antibody
Technique, Direct Human Pneumocystis carinii/*ISOLATION & PURIF
Pneumonia, Pneumocystis carinii/*DIAGNOSIS Sensitivity and Specificity
Specimen Handling/*METHODS Sputum/*MICROBIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).